In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT
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منابع مشابه
Prototype Foamy Virus ( PFV ) Gag nuclear localization – A novel pathway among 1 retroviruses 2 3
1 Prototype Foamy Virus (PFV) Gag nuclear localization – A novel pathway among 1 retroviruses 2 3 Erik Müllers, Kristin Stirnnagel, Sylvia Kaulfuß, and Dirk Lindemann* 4 5 Institut für Virologie, Medizinische Fakultät "Carl Gustav Carus", Technische Universität 6 Dresden, Dresden, CRTD / DFG-Center for Regenerative Therapies Dresden Cluster of 7 Excellence, Biotechnology Center, Dresden, German...
متن کاملDeoxynucleoside triphosphate incorporation mechanism of foamy virus (FV) reverse transcriptase: implications for cell tropism of FV.
Here, we investigated the pre-steady-state deoxynucleoside triphosphate (dNTP) incorporation kinetics of primate foamy virus (PFV) reverse transcriptase (RT) in comparison with those of HIV-1 and MuLV RTs. PFV RT displayed a drastic reduction in primer extension at low dNTP concentrations where HIV-1 RT remains highly active, indicating a low dNTP binding affinity in the case of PFV RT. Indeed,...
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Identification of drug resistant mutations is important in the management of HIV-1 infected patients. The aim of the current study was to evaluate drug resistance profile of RT gene and assess subtype among HIV-1 circulating strains and intensification of physician’s options for the best therapy. HIV-1 RNA of 25 sampleswas extracted from plasma and RT Nested- PCR was performed and the fin...
متن کاملMolecular dissection of the prototype foamy virus (PFV) RNA 5′-UTR identifies essential elements of a ribosomal shunt
The prototype foamy virus (PFV) is a nonpathogenic retrovirus that shows promise as a vector for gene transfer. The PFV (pre)genomic RNA starts with a long complex leader that can be folded into an elongated hairpin, suggesting an alternative strategy to cap-dependent linear scanning for translation initiation of the downstream GAG open reading frame (ORF). We found that the PFV leader carries ...
متن کاملEmergence of the H208Y mutation in the reverse transcriptase (RT) of HIV-1 in association with nucleoside RT inhibitor therapy.
OBJECTIVES The aim of the study was to determine whether mutations at RT codon 208 are associated with nucleoside RT inhibitor (NRTI) exposure, NRTI resistance patterns and HIV-1 subtype. METHODS Six thousand three hundred and fifty two genotypic resistance tests linked to a clinical database were analysed. RESULTS The prevalence of mutations at codon 208 was 6/2347 (0.3%) in treatment-naiv...
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ژورنال
عنوان ژورنال: Virology
سال: 2007
ISSN: 0042-6822
DOI: 10.1016/j.virol.2007.05.034